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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in lots of tumors, which leads to chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was identified to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a significant part in promoting cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually described. Two further splice variants have been described but comprehensive nucleotide sequence details is just not still out there.
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Blocking peptides are peptides that bind precisely into the focus on antibody and block antibody binding. These peptide typically consists of the epitope recognized because of the antibody.
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This guidebook outlines even more achievable optimization of mobile and immunoassay parameters to guarantee the best possible results are...
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This guideline outlines more probable optimization of cellular and immunoassay parameters to be sure the very best results are...
Antibodies certain to the blocking peptide no more bind to the epitope within the focus on protein. By evaluating the staining within the blocked antibody versus the antibody alone, one can see which staining is distinct.
Focus on Facts Cyclin E belongs to the highly conserved cyclin family members, whose associates are characterized by a dramatic periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to your temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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